PMC

Expression of wnt4a during reproductive duct development in zebrafish wild-type AB strain males. Alternate serial cross sections of wild-type males were stained by H&E to follow duct growth (see Figure S6) or prepared for in situ hybridization to reveal wnt4a expression, shown here. (A) Expression analysis showed that at 25 dpf, the duct deferens (cross section of the right duct shown here) lacked wnt4a expression. (B) At 25 dpf, however, wnt4a expression (→’s) appeared dorsal to the posterior vent, just anterior to the eventual connection of the fused duct deferens and the genital orifice. (C) At 35 dpf, the duct deferens continued to show little wnt4a expression. Insert shows the left and right duct deferens dorsal to the intestine. (D) At 35 dpf, wnt4a expression (→’s) appeared dorsal and lateral to the posterior vent, just anterior to the eventual connection of the fused ducti deferens and the genital orifice. (E) At 55 dpf, in a section just anterior to the fusion of the duct deferens to the vent, wnt4a expression (→) appeared dorsal to the posterior vent, just anterior to the connection of the fused ducti deferens and genital orifice. (F) At 55 dpf, in a section at the level of the connection of the fused duct and the vent, wnt4a expression (→) appeared just dorsal to the genital orifice. DD, ducti deferens; FDD, fused ducti deferens; GO, genital orifice; I, intestine; LDD, left duct deferens; PD, pronephric duct; RDD, right duct deferens; V, vent. Bar, 100 µm.

(a) Centrifugal microfluidic platform including: 1 the rotor securely affixed onto 2 an aluminum chuck, 3 the machined PEEK (PolyEtherEtherKetone) holding platform, and 4 the inserted microchip; (b) Design of the microchips (7 cm × 2.2 cm): C1 ion-exchange column, C2 restriction channel, C3 vent channel, R1 injection reservoir, R2 collection reservoir and chromatographic connection, R3 vent and chromatographic connection, E1 entry port, and E2 vent port of the injection reservoir, from [].

The connection between arterial line and pulmonary cannula is clamped. The pulmonary cannula is used as the vent.

A, sample bulb, B, reaction chamber; C, mercury manometer; I), U-tube cold trap at −78 °C; E, sieve separator; F, sieve pump; G, thermocouple gage; H. Toepler pump; I, gas burettes; J, recycle line; K, storage bulb; L, connections to vacuum pump (1); M, connection to vacuum pump (2); N, solenoid valves to control Toepler pump; O, connection to reaction chamber head for degassing base; P, thermocouple gage; Q, vent to air; R, connection to air pressure; ▲ arrow head showing direction of gas flow. ⊕ Stopcock.